MaCumBA


The FP7 project MaCuMBA focuses on the improvement of the isolation rate and the growth efficiency of marine microorganisms by applying innovative methods and procedures.
Within this project, MicroDish BV is involved in the screening and isolation of marine bacteria using the MicroDish Culture Chip. The development of high-throughput culture methods and the identification of culture conditions that enhance growth of slow growing microorganisms are additional goals of MicroDish BV.

 

HotZyme


This consortium of academic and industrial partners is dedicated to isolating thermostable hydrolytic enzymes for application in White Biotechnology. MicroDish BV is involved in the high-throughput screening and the creation of high value metagenomics libraries via the culture of novel extremophiles on the MDCC.

EvoTAR


EvoTAR is centered around the evolution and transfer of antibiotic resistance - a critical issue in global health. MicroDish BV is involved in screening for new microorganisms that are antibiotic resistant and analysis of microbial growth under extreme antibiotic stress.
This strong academic and commercial consortium is coordinated by the UMC Utrecht.

BIOMONAR


BIOMONAR has the aim to develop multiplexed nanoarray biosensors for selective and sensitive detection of environmental targets, i.e. pollutants and pathogens. The sensor platforms use customized microbes and microbial proteins to target specific molecules. MicroDish BV is contributing culture chip technology for screening and/or as a biosensor platform.

SAWA


SAWA is an innovation project in which mainly Dutch northern companies and knowledge institutes develop advanced sensors for monitoring of drinking water quality. The project is an initiative by Water Laboratory North (WLN), N.V. NOM, Water Company Groningen, Drenthe Water Company and Sensor Universe.
MicroDish will deploy miniaturized culture chips within water pipelines in order to speed up and automate the detection of fouling. MicroDish participates in the "regrowth" sub-project.

BE-Basic


BE-Basic is an international public-private partnership in the field of sustainable chemistry and ecology. MicroDish BV is participating in Flagship 7 - High Throughput Experimentation & (meta)genomic mining. The aim is to develop and apply high-throughput approaches and tools to explore and mine the metagenome, genetic material environmental samples. In addition this flagship aims to engineer and screen enzymes and other products for improved properties.
MicroDish BV supplies its direct partners with MicroDish Culture Chips and is involved in the development of assays for the high-throughput screening of (de)halogenases.

Surfixit


Surfixit is a consortium between the Wageningen University and three other companies under which MicroDish BV. The aim of the project is the generation of surface modifications for the application in micro-/nanotechnology-based analysis systems. Knowledge of the Wageningen University will be utilized by the industrial partners in order to develop new and innovative analysis technology.
Whithin this project, MicroDish BV is working on the (bio)chemical modification of the MicroDish Culture Chip surfaces for target-oriented applications.

Nano4Vitality


The economic and sustainability aspects of the production of small organic molecules by microorganisms is significant, both within the Netherlands and worldwide. MicroDish BV is collaborating with CSM/Purac to create high throughput screening systems for microbial strain improvement. Nanoscale detection reagents are being developed to work with the MDCC to achieve this goal.
This is being done within the umbrella of the N4V program (2008 to 2012).

Accelerating TB diagnostics


MicroDish BV, the Royal Tropical Institute in Amsterdam (KIT Biomedical Research) and Centre for Concepts in Mechatronics BV (Eindhoven) are collaborating to create rapid culture based diagnostics for tuberculosis (TB). This cooperation of culture chip (MicroDish), expertise in TB culture and diagnostics (KIT) and imaging (CCM) takes place within the MicroNed consortium.
The objective is to image TB during growth and rapidly assess the effect of drugs to enable rapid susceptibility testing. This project was initiated towards the end of 2008 and already has produced a publication - den Hertog AL, Visser DW, Ingham CJ, Fey FHAG, Klatser PR and Anthony RM (2010) Simplified Automated Image Analysis for Detection and Phenotyping of Mycobacterium tuberculosis on Porous Supports by Monitoring Growing Microcolonies. PLoS ONE 5(6)
 

Antibiotic Susceptibility Testing of Pathogens


A long standing collaboration between MicroDish BV and the Jeroen Bosch Hospital in s'Hertogenbosch (NL) is orientated towards the analysis of pathogenic microorganisms. This work includes slow growing fungi some of which (e.g. Aspergillus fumigatus) are developing drug resistance.
MDCC culture chip analysis is being used to accelerate diagnosis.
 

References


Ingham,CJ, van den Ende M, Pijnenburg, PC, Wever PC and Schneeberger PM (2005) Growth of microorganisms in a multiplexed format on a highly porous inorganic membrane (Anopore) App Environ Micro 71:8978-8981.

Ingham CJ, van den Ende M, Wever PC and Schneeberger PM(2006) Rapid antibiotic sensitivity testing and trimethoprim-mediated filamentation of clinical isolates of the Enterobacteriaceae assayed on a novel porous culture support Journal Medical Microbiology 55:1511-1519.

Ingham CJ, Sprenkels,A, Bomer JG, Molenaar D, van den Berg A, van Hycklama Vlieg JET and de Vos WM (2007) A highly subdivided microbial growth chip constructed on a porous ceramic support. PNAS 46:18217-18222.

den Besten HMW, Ingham CJ, van Hylckama Vlieg JET, Beerthuyzen M Zwietering MH and Abee, T (2007) Use of a direct imaging method to quantitatively assess the effects of mild and severe salt stress on population heterogeneity of Bacillus cereus ATCC 14579 App Environ Micro 73:4797-4804.

Gefen O and Balaban NQ (2008) The Moore's Law of microbiology - towards bacterial culture miniaturization with the Micro-Petri chip. Trends in Biotechnology 26:345-347.

Dittrich PS (2008) Research Highlights. Lab on a Chip 8:195-197.

Ingham CJ, Ben Ayad A, Nolsen K and Mulder B (2008) Growth and drug sensitivity testing of Mycobacterium tuberculosis complex on a novel porous ceramic. International Journal of Tuberculosis and Lung Disease 12: 645-650.

Ingham CJ and Hycklama Vlieg JET (2008) MEMS and the microbe. Lab on a Chip. 8:1604-1617.

Ingham CJ, Bomer J, Sprenkels A, van den Berg, A, de Vos WM, van Hylckama Vlieg JET (2010) High-resolution microcontact printing and transfer of massive arrays of microorganisms on planar and compartmentalized nanoporous aluminum oxide. Lab on a Chip, 10:1410-1416.

den Hertog AL, Visser DW, Ingham CJ, Fey FHAG, Klatser PR, Anthony RM (2010). Simplified automated image analysis for detection and phenotyping of Mycobacterium tuberculosis on porous supports by monitoring growing microcolonies. PLoS ONE 5(6).

Ingham CJ, ter Maat J, de Vos WM (2011). Where nano- meets bio-; the many uses of porous aluminium oxide in biotechnology. Biotech Adv DOI: 10.1016/J.biotechadv.2011.08.005.

Ingham CJ and de Vos WM (2011) Moore's Law and Microbiology. The Microcanon. Published Veen Magazines. Dutch.

ter Maat J, Regeling R, Ingham CJ, Weijers CA, Giesbers M, de Vos WM, Zuilhof H (2011) Organic modification and subsequent biofunctionalization of porous anodic alumina using terminal alkynes. Langmuir 27:13606-13607.

Ingham CJ, Boonstra S, Levels S, de Lange M, Meis JF, Schneeberger PM (2012) Rapid susceptibility testing and microcolony analysis of Candida spp cultured and imaged on porous aluminium oxide. PLoS ONE 7(3):e33813.

Ingham CJ and Schneeberger PM (2012) Microcolony imaging of Aspergillus fumigatus treated with echinocandins reveals both fungistatic and fungicidal activities. PLoS ONE in press.